QUANTITATIVE RAPID IDENTIFICATION OF ANTIGENS AND ANTIBODIES

Platelet antigens and antibodies in multitransfused patients

Abstract Background and Objectives Following incompatible blood transfusions, anti-HLA and  anti- HPA antibodies may develop and cause various disorders such as post-transfusion purpura, platelet refractoriness, and thrombocytopenia leading to bleeding. The aim of this study was to investigate platelet antigens and antibodies in multi-transfused patients.   Materials and Methods In this desc...

Identification of antigens of Mycobacterium tuberculosis using human monoclonal antibodies.

Pleural tuberculosis constitutes a human model of local protective immunity to mycobacterial infection as the disease is usually self-limited and recurrent pleurisy is rare. To identify potentially protective antigens of Mycobacterium tuberculosis, 37 human pleural fluid B cell clones were established using EBV and their supernatants assayed by ELISA and Western blot for antibody reactivity wit...

Identification of human sperm antigens to antisperm antibodies.

We have successfully applied SDS (sodium dodecyl sulfate) gel/protein blot radioimmunobinding method to identify the molecular size of sperm antigens that elicit antisperm antibodies from patients with unexplained infertility. Following the transfer of renatured proteins from SDS gel of human sperm extract onto nitrocellulose strips, the radioimmunobinding was performed by incubating the strips...

Identification of antibodies to acidic antigens by counterimmunoelectrophoresis.

Direct identification of unexpected serum antibodies against red cell antigens.

A new method to detect the identity of unexpected antibodies in the sera of prospective transfusion recipients is presented. The method is based on a macroscopic hemagglutination inhibition assay. In this assay, incubation of phenotypically defined test red blood cells with patient sera containing non-agglutinating antibodies to one or more specific antigen systems block monoclonal IgM inductio...